Modulation of Gene Expression by Human Cytosolic tRNase ZL through 5′-Half-tRNA (original) (raw)
Figure 4
Δ30 tRNase ZL exists primarily in the cytosol.
(A) Western blotting for human tRNase ZL from the 293 cells. The recombinant full-length and Δ30 tRNase ZL standards were generated from intein-fusion proteins. The membrane was probed with antibodies raised to a human tRNase ZL peptide (amino acid 812–826). (B) Western analyses for human tRNase ZL in the subcellular fractions. The cytosolic (F1), membrane/organelle (F2), nuclear (F3), and cytoskeletal (F4) fractions were prepared from the 293 cells. (C) Total protein samples from the 293 cells that were transfected with the tRNase ZL expression plasmid (+ZL) or with the tRNase ZL siRNA (−ZL) were subjected to western analyses.