A Novel In Vitro Multiple-Stress Dormancy Model for Mycobacterium tuberculosis Generates a Lipid-Loaded, Drug-Tolerant, Dormant Pathogen (original) (raw)
Figure 5
Decrease in buoyant density of Mtb cells subjected to multiple-stress.
Mtb cells subjected to the multiple-stresses were placed on the preformed gradient and centrifuged at 400 g for 20 min. The center tube is a 3 day cell sample mixed with density marker (M) beads. Percoll gradients were self-formed by centrifugation from a starting solution with a density (ρ) of 1.0925 gm/ml. The densities of selected bead layers (ρ, in gm/ml) are given on the right, and the positions of one ml fractions collected for analyses are at the left. Numbers below the tubes indicate the number of days under multiple-stress.