Human Mesenchymal Stem Cells Self-Renew and Differentiate According to a Deterministic Hierarchy (original) (raw)
Figure 2
HUCPVCs survive and regenerate damaged mesenchymal tissues in vivo.
HUCPVCs were synthetically active and survived at least six weeks in vivo when implanted into the intrafemoral space of NOD-scid mice as observed by the presence of GFP-labeled cells among mouse cells flushed from the marrow into culture (B,C). When the distal ends of the femurs were analyzed by micro-computed tomography (µCT), it was determined that these cells produced significantly more bone mineral density (BMD) than sham controls at 2,4 and 6 weeks (A, values are means±s.d.). µCT analysis and Masson's Trichrome stained longitudinal sections of injected femurs showed that HUCPVCs induced significantly more repair of bone and cartilage at 2 weeks (D,E) compared to sham controls (F,G). HUCPVC populations from a total 6 different cords were employed to generate this data and that shown in Figure 3.