Multipolar Spindle Pole Coalescence Is a Major Source of Kinetochore Mis-Attachment and Chromosome Mis-Segregation in Cancer Cells (original) (raw)
Figure 3
Both multipolar prometaphase and bipolar metaphase CIN cells possess multiple centrosomes.
The figure shows examples of multipolar prometaphase (A) and bipolar metaphase (B) CIN cells immunostained for α-tubulin, kinetochores, and γ-tubulin. Images shown were obtained by merging maximum intensity projections of either α-tubulin and CREST (kinetochores) images (left column) or α-tubulin and γ-tubulin images (right column). A. Most multipolar prometaphase cells (exact percentages shown at the bottom right corner of the right panels) exhibited γ-tubulin staining at all spindle poles. B. Examples of bipolar metaphase CIN cells with multiple centrosomes at a single spindle pole (arrows point at γ-tubulin-stained dots). Scale bar, 5 µm. C. Frequencies of bipolar metaphase/early anaphase CIN cells possessing multiple centrosomes (as visualized by γ-tubulin staining) at a single spindle pole.