The Wnt Receptor, Lrp5, Is Expressed by Mouse Mammary Stem Cells and Is Required to Maintain the Basal Lineage (original) (raw)
Figure 2
Lrp5 is Expressed by Basal Mammary Epithelial Cells.
A) BALB/c virgin MECs were stained with CD24, CD49f, and Lrp5 prior to FACS analysis. MECs were gated for positive (blue) and negative (red) Lrp5 expression and the subsequent CD24/CD49f profile of the populations were overlaid. B) Basal MECs were gated based on the magnitude of CD24/CD49f expression level, as CD24low/CD49flow (1, red) or CD24high/CD49fhigh (2, black). The relative Lrp5 expression levels of each population were then overlaid as histograms. C) Basal, Luminal, Lrp5+, Lrp5-, and whole population (WP) MEC fractions (gates show in A) were isolated by FACS and subsequently stained for K5 and K8 to confirm their purity and cellular phenotype.