The Wnt Receptor, Lrp5, Is Expressed by Mouse Mammary Stem Cells and Is Required to Maintain the Basal Lineage (original) (raw)
Figure 5
Lrp5-High Cells are Enriched for Stem Cell Activity.
A) Mammary epithelial cells were isolated from 10-week, virgin BALB/c mice, stained for Lrp5 and FACS sorted, as described above. Lrp5 high (red) and negative (green) MECs were then transplanted into cleared fat pads of 3-week BALB/c recipient mice. Following 8 weeks, mammary glands were harvested, carmine stained, and scored for primary outgrowths, as described above. Transplantations of the MRU sub-population (black), in addition to the FACS sorted, unfractionated population (Whole Population, gray) served as controls. Left panel is a representative CD24/CD49f FACS profile, depicting overlaid Lrp5- (red), Lrp5 low (blue), and Lrp5 high (green) populations. B) Estimation of stem cell frequencies for each transplanted population. Stem cell frequencies, fold enrichment, and p values were calculated using limdil software (http://bioinf.wehi.edu.au/software/limdil).