Effects of S1 Cleavage on the Structure, Surface Export, and Signaling Activity of Human Notch1 and Notch2 (original) (raw)
Figure 7
Activation of S1-cleavage resistant Notch receptors by ligands of the Delta and Jagged families.
(A) Isogenic U2OS flp-in cell lines that express N1-Gal4 or N1-Gal4-LO receptors under the control of tetracycline were transfected with a Gal4 responsive luciferase reporter plasmid and co-cultured with OP9 or OP9-Delta-like-1 feeder cells in the presence of tetracycline for 24 hr. Dual luciferase activities were measured in whole cell lysates. In (B), the experiment is identical to that in (A), except that 3T3 or 3T3-Jagged2 feeder cells were used. (C) Isogenic U2OS flp-in cell lines that express N2/N1-Gal4 or N2/N1-Gal4-LO receptors under the control of tetracycline were transfected with a Gal4 responsive luciferase reporter plasmid and co-cultured with OP9 or OP9-Delta-like-1 feeder cells in the presence (+) or absence (−) of tetracycline for 24 hr. Dual luciferase activities were measured in whole cell lysates. In (D), the experiment is identical to that in (C), except that 3T3 or 3T3-Jagged2 feeder cells were used.