Diversity and Strain Specificity of Plant Cell Wall Degrading Enzymes Revealed by the Draft Genome of Ruminococcus flavefaciens FD-1 (original) (raw)
Figure 5
Proportions of cellulases, enzymes cleaving non-cellulosic plant cell wall polysaccharides (including carbohydrate esterases) and other predicted ORFs among the total cellulosome-associated genes and the up-regulated cellulosome-associated ORFs.
Up-regulated genes are those dockerin-containing ORFs that have fold changes of 2-fold or greater when grown on cellulose. For the purposes of this work, the putative cellulases include any ORF containing glycoside hydrolase (GH) families 5, 8, 9, and 48. The enzymes cleaving non-cellulosic plant cell wall polysaccharides (mainly hemicellulases) include ORFs containing GH families 10, 11, 16, 26, 43, 44, 53, 74 105, some subfamilies of GH5, all families of polysaccharide lysases (PL) and carbohydrate esterases (CE). ORFs that did not have any significant hits in the database are grouped as “unknown,” and ORFs that do not fall into any of the previous categories are grouped as “other.” Putative β-glucosidases and β-xylosidases were ORFs containing sequences consistent with GH family 3.