Whole Genome Amplification and De novo Assembly of Single Bacterial Cells (original) (raw)
Figure 1
Sequencing coverage after WGA of individual Prochlorococcus cells.
A) Average fold-coverage within 100 bp windows was determined for SAG A from the alignment of 454-FLX read data (blue) and Illumina read data (red) to the Prochlorococcus MED4 reference genome. B) Genome recovery for individual Prochlorococcus cells, SAG A and SAG B, as a function of sequencing effort for Illumina libraries (35 nucleotide read lengths). Each datapoint represents the mean genome recovery from at least two reference-guided assemblies. Standard deviations are too small to be visible on the figure.