Hypoxia and TGF-β Drive Breast Cancer Bone Metastases through Parallel Signaling Pathways in Tumor Cells and the Bone Microenvironment (original) (raw)
Figure 7
HIF-1α knockdown decreases tumor angiogenesis at sites of bone metastases in mice.
(A) Representative sections from mice with bone metastases stained for the endothelial cell marker CD31. Arrows indicate CD31+ vessels. Scale bar equals 50 µm (B) The number of vessels in bone metastases was counted in three non-overlapping fields at 200×magnification per mouse (n = 3 per group). Results are expressed as the mean ± SEM number of vessel per tumor area. * _P<_0.05, ** _P<_0.01, **** _P<_0.001, using a one-way ANOVA with a Newman-Keuls multiple comparison test.