A Microscope Automated Fluidic System to Study Bacterial Processes in Real Time (original) (raw)
Figure 3
SodA-mcherry induction in cells containing inclusion bodies during MV injection.
A) Measurement of SodA induction after Methyl Viologen injection and removal (indicated as grey shade) in cells carrying the SodA-mcherry fusion. Each colored curve represents the kinetics of expression of a given single cell B) Measurement of SodA induction in cells that carry polar foci of the protein aggregation marker IbpA-YFP. C) Sequence of images showing several rounds of cell division from a single mother cell carrying a polar IbpA-YFP polar focus; Top: phase images. Bottom: overlay of YFP (magenta) and mCherry fluorescent signals. Cell contours retrieved by the annotation software are added for clarity (yellow cells containing IbpA-YFP clusters and green cells not containing the clusters).