A New Paradigm for MAPK: Structural Interactions of hERK1 with Mitochondria in HeLa Cells (original) (raw)
Figure 2
hERK1-GST pulldown assay. GST-hERK1 or GST-null recombinant proteins immobilized on GSH-agarose were incubated overnight with a mitochondrial extract.
Beads were extensively washed and eluted proteins were run on SDS-PAGE. Proteins were stained with Coomasie Brilliant Blue G-250. Molecular weight markers displayed on the left. GST-hERK1 and GST-null recombinant proteins highlighted on the right. Numbers on the right side of the bands recovered in GST-hERK lane represent proteins identified by mass spectrometry and shown in Table 1.