Filamins Regulate Cell Spreading and Initiation of Cell Migration (original) (raw)
Figure 6
FLNa* but not FLNa*Δ19–21 rescues motility of FLNabKD cells.
A and B) FLNabKD cells were transfected with FLNa*-GFP and assessed in time-lapse migration assays. Re-expressing cells were identified by staining for FLNa. Mean % of non-motile cells (A) and mean cell speed (B) in untransfected and FLNa-re-expressing FLNabKD cells. Values ±SEM from at least 4 independent experiments: sample size FLNabKD = 75; FLNabKD+FLNa* = 9). C and D) FLNabKD cells were transfected with FLNaΔ19–21 and assessed in time-lapse migration assays. Cells expressing FLNaΔ19–21 were identified by staining for FLNa. Mean cell area was measured by manually rendering the cell contour in the phase contrast (C) and motility assessed as described previously (D). Values ± SEM from at least 4 independent experiments: (sample size FLNabKD = 94; FLNabKD+FLNa*Δ19–21 = 10). 1 pixel2 = 1.664 µm2.