Japanese Encephalitis Virus Induce Immuno-Competency in Neural Stem/Progenitor Cells (original) (raw)
Figure 1
Upregulation of costimulatory molecules and MHC class I in Nestin-positive cells in SVZ during JEV infection.
Brain cryosections from control and JEV infected animals were stained with antibodies against CD40, CD80, CD86, and MHC class I molecules and Nestin. Isotype staining using rat IgG2aκ (for costimulatory molecules and MHC class I) and normal mouse serum (NMS; for Nestin) was performed on both control (A) and JEV infected brain sections (B). Co-localization of Nestin (red) with the costimulatory molecules and MHC class I (green) was performed using confocal microscopy. Isotype staining on both control and JEV infected sections show no detectable fluorescence (A–B). The Nestin +ve cells show a distinctive morphological change following infection from process bearing cells to round/oval shaped cells. Nestin positive cells in control sections show very less co-localization with all the costimulatory molecules and MHC class I (C, E, G, I). JEV infected SVZ show complete and increased co-localisation of Nestin positive cells with CD40 (D), CD80 (F), CD86 (H), and MHC class I (J). Scale bar corresponds to 20 microns.