Combined Bezafibrate and Medroxyprogesterone Acetate: Potential Novel Therapy for Acute Myeloid Leukaemia (original) (raw)
Figure 5
BEZ induction of ROS is concentration dependent and correlates with cell killing.
BEZ induced reactive oxygen species (ROS) were measured at 14 hours by staining with carboxy-H2DCFDA and analysis by flow cytometry. (A) Histogram shows representative results from HL60 cells. (B) ROS induction was measured in K562 and KG1a cells after 14 hrs treatment with different BEZ concentrations (mean±sem from N = 4 experiments). (C) ROS induction with 0.5 mM BEZ for 5 myeloid cell lines is shown from a minimum of N = 3 experiments for each cell line. Mean is indicated by black bar. (D) % viable KG1a cells at day 10 compared to controls plotted against the % cells positive for ROS at increasing BEZ concentrations. Statistics *p<0.01.