Genome-Wide Identification of Schistosoma japonicum MicroRNAs Using a Deep-Sequencing Approach (original) (raw)
Figure 3
Identification and properties of S. japonicum miRNAs.
(A) Length distribution of all identified S. japonicum miRNAs. The left and right Y-axes indicate the number and percentage of miRNAs, respectively. (B) Cumulative length distributions of miRNA precursors from S. japonicum and other bilaterian animals. The size distribution of S. japonicum miRNA precursors is similar to that of other bilaterian animals, including C. elegans (cel), Schmidtea mediterranea (sme), D. melanogaster (dme), Fugu rubripes (fru), mice (mmu), and humans (hsa). (C) The sequences and numbers of sequencing reads matching the sja-miR-2e hairpin. The sequence of the sja-miR-2e hairpin is displayed above the bracket-notation of its predicted secondary structure, as determined by RNAfold. Sequenced small RNAs from immature schistosomula (SC) and adult worms (AW) that map to the hairpin are aligned below, with the number of reads shown on the right. Both miR-2e-5p and the conserved miR-2e-3p were designated as reciprocal miRNA and miRNA* species and are indicated in red and in blue, respectively. (D) Relative expression levels of S. japonicum miRNAs, as indicated by fold enrichment through normalizing the frequency of sequencing reads from the AW and SC samples.