Notch2 Signaling Sensitizes Endothelial Cells to Apoptosis by Negatively Regulating the Key Protective Molecule Survivin (original) (raw)
Figure 4
Notch2 signaling downregulates apoptosis mediators.
(A) A schematic representation of apoptosis-related transcripts repressed (red) or induced (green) by N2ICD or TNF determined by a dedicated PCR array. Means are shown as fold changes compared to AdGFP or untreated ECs, respectively. (B) Validation of transcripts regulation by qRT-PCR in ECs transduced with AdN2NICD (moi 20 and 40) in comparison to controls (non infected (NI) and AdGFP) (B–C). (D) Dose-response effect of AdN2ICD on Survivin protein expression by western blot. Results are representative of 5 independent experiments. *p<0.05 versus controls.