Patterned Neuroprotection in the Inpp4awbl Mutant Mouse Cerebellum Correlates with the Expression of Eaat4 (original) (raw)
Figure 5
Purkinje cell loss coincides with the appearance of climbing fibers in the developing molecular layer.
Panels d, e, f show climbing fibers labeled by anti-vGlut2 (red) and Purkinje cells labeled by anti-calbindin (green) in wild type controls at P3, P5 and P7. Climbing fibers can be seen to extend to the limit of the developing molecular layer (ml) inferior to the external granule cell layer (egl). In age-matched mutant littermates (panels a,b,c) Purkinje cells (pcl) have an unusual appearance beginning at P5, multiple thin dendrites are seen with a prominent staining rounded soma (arrow). Scale bar indicates 25 microns.