Bordetella pertussis Commits Human Dendritic Cells to Promote a Th1/Th17 Response through the Activity of Adenylate Cyclase Toxin and MAPK-Pathways (original) (raw)
Figure 6
Effects of kinase inhibition on Th polarization.
A MDDC, either untreated or treated with BpWT or BpCyaA− in the absence or presence of p38 inhibitor (SB203580), ERK1/2 inhibitor (PD98059) or PI3K inhibitor (LY294002) for 48 h, were co-cultured with purified T cells. On day 12, supernatants were collected and secreted cytokines were measured by ELISA. Results of four independent experiments performed with MDDC and T cells obtained from different donors are expressed as the percent of change of cytokines (IFNγ, IL-17 and IL-5) with respect to the corresponding stimulus in the absence of inhibitors. Mean ± SE of cytokine production (pg/ml) in MDDC not treated with inhibitors was for BpWT: IFNγ = 9013±1828; IL-17 = 900±68; IL-5 = 71±19; for BpCyaA−: IFNγ = 9834±1225; IL-17 = 423±94; IL-5 = 21±5. * p<0.05 vs control, calculated from the raw data.