S100a9 Knockdown Decreases the Memory Impairment and the Neuropathology in Tg2576 Mice, AD Animal Model (original) (raw)
Figure 1
S100a9 was induced in the brains of CT-Tg, Tg2576 mice and Human AD patients.
(A) mRNA levels of S100a9 were measured in the cortex and hippocampus of CT-Tg and age-matched WT mice brains by RT-PCR. Actin was used as a loading control. The protein level of S100a9 was checked by western blotting. Tubulin was used as a loading control. (B) S100a9 expression was normalized with that of tubulin for quantification (n = 5). Results are presented as the means ± SEM. *P <0.05, **P<0.01 by Student's _t-_test. (C) Immunoreactivities of S100a9 were examined in the cortex, hippocampus [CA3, dentate gyrus (DG)] of 11-month-old CT-Tg, of 11-month-old Tg2576 and of human AD brains compared with normal age-matched brains. Scale bar represents 100 µm.