Light and Electron Microscopy Study of Glycogen Synthase Kinase-3β in the Mouse Brain (original) (raw)
Figure 4
Electron microscopy images of GSK3β labeling in the piriform cortex.
A) Two adjacent labeled neurons. B) Detail of neurons in A. GSK3β labeling is located in the rough endoplasmic reticulum (outlined arrows) and free ribosomes (outlined stars). C) Detail of neurons in A showing labeled mitochondria (black arrows), abundant labeled free ribosomes (outlined stars), and also labeled rough endoplasmic reticulum (outlined arrows). Note also the labeling in dendrites (d). D) Another labeled neuron with strong cytoplasmic staining. Dendrites (d) are also labeled. E) Detail of neuron in D showing strongly labeled rough endoplasmic reticulum (outlined arrow), free ribosomes (outlined stars) and also labeling on the outer surface of the mitochondria (black arrows). Note also unlabeled myelinated axons (black star). F) High magnification image of two dendrites (d). GSK3β staining is conspicuously present on the postsynaptic density (outlined arrowheads). Cytoplasmic elements including the outer membrane of mitochondria (black arrows) are also labeled. However, no labeling is present in axon terminals (at). [N: nucleus; Scale bars: 4 µm in A–D; 1 µm in B–C and E–F]