Flagellin-Induced Corneal Antimicrobial Peptide Production and Wound Repair Involve a Novel NF-κB–Independent and EGFR-Dependent Pathway (original) (raw)
Figure 9
Flagellin upregulates EGFR signaling pathways in the epithelia of healing porcine corneas.
Epithelial cells in healing porcine corneas treated with wild type (PF), L94A (LF) flagellin, or medium alone (CT), were collected 48 hrs post wounding, lysed in RIPA buffer and sonicated. Equal amounts of cell lysates from 2 of 5 corneas (randomly picked) from each group were immunoblotted with antibodies against phospho-EGFR, AKT or ERK1/2. The immunoreactivities were stripped off and the same membranes were reprobed with antibodies against cellular EGFR, AKT or ERK2, respectively, for proper protein loading.