Myelin Activates FAK/Akt/NF-κB Pathways and Provokes CR3-Dependent Inflammatory Response in Murine System (original) (raw)
Figure 4
Effect of myelin on NF-κB activation.
(A) Bone marrow-derived macrophages were incubated with myelin for indicated time and phosphorylation/degradation of IκB-α were assessed by western blot. (B) Effect of NF-κB inhibitor BAY 11-7082 on myelin-induced IκB-α phosphorylation and degradation. (C) Myelin-induced p65 translocation in macrophages detected by immunofluorescent staining. p65 was stained red and nuclei were stained with DAPI (blue) (Scale bar = 10 µm). (D) Sphingomyelin (25 µg/mL) had no effect on IκB-α phosphorylation and degradation macrophages. (E) MBP (1 µg/mL) had no effect on IκB-α phosphorylation and degradation in bone marrow-derived macrophages. The inhibitor was used for 30-minute pre-incubation before myelin incubation. Phosphorylation levels were normalized to non-phosphorylated total proteins. Similar results were obtained from three independent experiments.