The C. elegans D2-Like Dopamine Receptor DOP-3 Decreases Behavioral Sensitivity to the Olfactory Stimulus 1-Octanol (original) (raw)
Figure 6
DOP-3::RFP expression is not seen in the sensory neurons that detect octanol.
The ASH sensory neurons are the primary sensors of 100% octanol when animals are assayed on food, and only ASH is used to detect diluted octanol both on and off food [19]. The integrated transgene vsIs33 encodes 13,035 base pairs of dop-3 genomic DNA, including 9,955 base pairs of upstream promoter sequence and extending into the fourth coding exon [25]. This sequence is fused to the mRFP1 red fluorescent protein [25], [75]. DOP-3::RFP expression was not observed in ASH (marked by osm-10::gfp), or the other octanol-detecting neurons, AWB (marked by str-1::gfp) or ADL (marked by gpa-15::gfp). Weak expression was often observed in ASK (marked by gpa-15::gfp). Scale bar = 20 µm.