The Extracytoplasmic Function Sigma Factor SigV Plays a Key Role in the Original Model of Lysozyme Resistance and Virulence of Enterococcus faecalis (original) (raw)
Figure 3
Complementation assay under lysozyme treatment.
The parental strain E. faecalis JH2-2 (1), and its derivative mutants Δ_sigV_ (2), SAS pMSP3535 (3), and SAS pMSP3535-sigV (4) were grown on LB solid medium without lysozyme (A) or supplemented with 20 mg/ml of lysozyme (B). Five µl of 10−2 dilution of overnight cultures adjusted to OD600 = 1 in physiological water were plated on solid medium, incubated at 37°C for 48 hours and photographed.