Sensitive Detection of Gene Expression in Mycobacteria under Replicating and Non-Replicating Conditions Using Optimized Far-Red Reporters (original) (raw)
Figure 6
Detection of fluorescent reporter in M. tuberculosis H37Rv.
FP expression plasmids were transformed into M. tuberculosis and colonies isolated on hygromycin-containing plates. (A) Expression of several fluorescent reporters gave rise to coloured colonies. (B) Microscopy of fluorescent mCherry reporter driven by either Phsp60 or Psmyc. Scale bar is equal to 10 µm.