Identification of Genes Involved in Polysaccharide-Independent Staphylococcus aureus Biofilm Formation (original) (raw)
Figure 4
Time course of autolysis in selected biofilm mutants.
Planktonic cultures of seven transposon mutants harboring plasmid pAJ22, which expresses cytoplasmic β-galactosidase, were grown for 72 hrs. Every 12 hr during the time course samples were removed from cultures and β-galactosidase activity in cell free supernatants was measured (reported in Miller units). SH1000 with pAJ22 was included as a control (shown as black squares). Results shown were the average of two independent experiments done in triplicate and error bars show standard deviation.