The Transcription Factor Cux1 Regulates Dendritic Morphology of Cortical Pyramidal Neurons (original) (raw)
Figure 2
Down-regulation of Cux1 by RNAi increases the dendritic complexity of cortical pyramidal neurons.
(A–B) RT-PCR analysis of Cux expression level following RNAi treatments. Two specific RNAi each designed to different regions of ORF for Cux1 and Cux2, Cux1/2-RNAiA and -RNAiB, could result in the down-regulation of their expression levels. Representative images are semi-quantitative PCR. Quantitative results are measured by real-time PCR. Data shown are mean ± SEM (n = 3 independent experiments). * P<0.05, ** _P_<0.01, paired _t_ test. (C–E) Increase of pyramidal neuron dendritic complexity after transfection of Cux1-RNAi plasmids. Representative cells are shown in (C). Scale bars: 50 µm. Data shown are mean ± SEM (n≥30 for each condition). ** _P_<0.01, Student's _t_ test. (F–G) Dendritic morphology of pyramidal neurons after transfection of Cux2-RNAi plasmids. Data shown are mean ± SEM (n≥30 for each condition). _P_>0.05, Student's t test.