The Transcription Factor Cux1 Regulates Dendritic Morphology of Cortical Pyramidal Neurons (original) (raw)
Figure 5
Modulation of p27 by RNAi-treatment of Cux1.
(A–B) Increase of p27 expression level by transfection of Cux1-RNAi plasmids into cultured cortical neurons, as shown by RT-PCR (A) and Western blotting (B). Representative images in (A) are semi-quantitative PCR. Quantitative results in (A) are measured by real-time PCR. Data shown are mean ± SEM (n = 3 independent experiments for each condition). * P<0.05, paired t test. (C–D) Reducing the expression of p27 by RNAi simplified the dendritic complexity of cortical pyramidal neurons in culture. Data shown are mean ± SEM (n≥30 for each condition). * P<0.05, ** P<0.01, Student's t test. (E–F) Time-course of dendritic complexity change after p27-RNAiA treatment. Three time points were examined: DIV4, 5, and 6. Data shown are mean ± SEM (n≥30 for each condition). * P<0.05, ** P<0.01, Student's t test. (G–H) Rescue of Cux1-RNAiA's effect on dendritic complexity of pyramidal neurons by p27-RNAiA. Data shown are mean ± SEM (n≥30 for each condition). ** P<0.01, ANOVA SNK test.