MicroRNA 128a Increases Intracellular ROS Level by Targeting Bmi-1 and Inhibits Medulloblastoma Cancer Cell Growth by Promoting Senescence (original) (raw)
Figure 4
Growth inhibition signaling pathways modulated by miR-128a.
A) Western blot analysis showed increased p16 expression in Daoy cells transfected with miR-128a compared to that of the control vector, pVETL. The level of p16 expression was completely inhibited in Daoy cells that were transfected with the Bmi-1 vector alone while modest inhibition was observed in cells co-transfected with miR-128a. B) Decreased E2F1 activity in Daoy cells transfected with miR-128a as measured by luciferase reporter assays. (p<0.01)