Mutually Positive Regulatory Feedback Loop between Interferons and Estrogen Receptor-α in Mice: Implications for Sex Bias in Autoimmunity (original) (raw)
Figure 6
The IFN and E2-signaling cooperate to activate transcription of genes.
(A) Sub-confluent cultures of WT276 cells in a 6-well plate were transfected with ERE-luc-reporter plasmid (2 µg) along with pRL-TK (0.2 µg) plasmid using FuGENE 6 transfection reagent. 24 h after transfections, cells were either left untreated (column 1), treated with E2 (column 2), IFN-α (column 3) or E2 and IFN-α (column 4). 40–45 h after transfections, cells were processed for dual luciferase activity. Result are mean values of triplicate experiments and error bars represent standard deviation (p value is 0.006). (B) Sub-confluent cultures of WT276 cells in a 6-well plate were transfected with ISRE-luc-reporter plasmid (2 µg) along with pRL-TK (0.2 µg) plasmid using FuGENE 6 transfection reagent. 24 h after transfections, cells were either left untreated (column 1), treated with E2 (column 2), IFN-α (column 3) or E2 and IFN-α (column 4). 40–45 h after transfections, cells were processed for dual luciferase activity.