Bryostatin Modulates Latent HIV-1 Infection via PKC and AMPK Signaling but Inhibits Acute Infection in a Receptor Independent Manner (original) (raw)
Figure 2
Bryostatin ablates R5-tropic HIV infection.
Magi cells (CD4+/CCR5+) were infected with 100 ng/ml p24 equivalent HIV recombinant NLENYU2 virus expressing YFP. (A) Productive viral infection was monitored semi-quantitatively on 3rd and 5th dpi. (B) GFP-positive cells per 10 fields were counted in HIV-infected cultures and plotted as mean. (C) Dose dependent anti-HIV effect of bryostatin was monitored by p24 assay. (D) p24 levels in the culture supernatants after treatment with bryostatin (25 ng/ml) and other compounds on 3rd and 5th day post infection are shown. Sodium salicylate (1 mM) was used as a negative control in each set of experiments. Degree of significance for bryostatin treatment was relative to virus control, * p<0.05, # p<0.001. (n = 4).