Blockade of TRPM7 Channel Activity and Cell Death by Inhibitors of 5-Lipoxygenase (original) (raw)
Figure 1
Inhibitors of 5-LOX block TRPM7-mediated cell rounding.
(A) Application of the 5-LOX inhibitors NDGA (10 µM), AA861 (10 µM), and MK886 (10 µM) to 293-TRPM7 expressing cells reduced cell rounding. In contrast, treatment of 293-TRPM7 cells with the cyclooxygenase inhibitor indomethacin (10 µM), 12-LOX inhibitor baicalein (5 µM), 15-LOX inhibitor PD146176 (2 µM), and the 5-LOX inhibitor zileuton (50 µM) did not block TRPM7-induced cell rounding. (B) Quantification of the degree of cell rounding under the conditions depicted in (A). Values are mean ± standard deviation of at least three independent experiments. A χ2 test was employed to assess differences in cell rounding between 293-TRPM7 cells treated with the different inhibitors. An asterisk indicates treatments that produced a decrease in cell rounding that was significantly different from 293-TRPM7 cells grown in tetracycline.