Dendritic Cells in Chronic Mycobacterial Granulomas Restrict Local Anti-Bacterial T Cell Response in a Murine Model (original) (raw)
Figure 5
CD4+ T cells maintain contact with CD11c-EYFP+ cells in both acute and chronic granulomas.
A, Quantitative analysis of CD4+ T cell contact with CD11c-EYFP+ cells in granulomas from three- and ten-week liver sections. All CD4+ T cells per granuloma section were determined to be in contact (green bars), or not in contact (white bars) with CD11c-EYFP+ cells. For each time point, liver sections from 3 independent mice were used, and 10–15 lesions per section were analyzed. Data are represented as mean +/− SEM, P<0.0001. B, Fluorescent images demonstrating CD11c-EYFP contact with CD4+ cells. Granulomas are outlined with white dashed lines at 1000× magnification and colors shown depict CD11c-EYFP (green cells), anti-CD4 Alexa 647 (red cells), and DAPI nuclear stain (blue).