Activated Notch1 Target Genes during Embryonic Cell Differentiation Depend on the Cellular Context and Include Lineage Determinants and Inhibitors (original) (raw)
Figure 7
Notch1 target genes validated by qPCR.
The microarray results were validated by qPCR using ESCs, ESCe, ESCm and Mesoderm. All conditions were tested also in the absence or presence of cycloheximide (+CHX). Oct4 (last gene; also known as Pou5f1) is included as negative non-regulated control. (A) Mean values of relative expression (% of Gapdh expression, Log2 transformed) shown as a heatmap in the absence (−) or presence (+) of OHT. (B) Inductions by activated Notch1 were calculated from relative expression values and were Log2 transformed. Thus, blue areas indicate down-regulation and red regions show up-regulation. *) indicates low level expression of Myf5 near detection limit resulting in artificially high induction values that are not reliable.