Activated Notch1 Target Genes during Embryonic Cell Differentiation Depend on the Cellular Context and Include Lineage Determinants and Inhibitors (original) (raw)
Figure 8
Regulatory transcriptions factors involved in differentiation are induced by activated Notch1 in the absence of protein synthesis.
(A–H) The microarray results were validated by qPCR in ESC cultured in self renewal (ESCs), ectodermal (ESCe), or mesodermal (ESCm) culture medium and in mesodermal cells (Mes.). All conditions were tested in the absence (−) or presence (+) of CHX. Relative expression is shown for untreated (blue bars) and OHT treated samples (red bars) compared to Gapdh. The calculated inductions are indicated by green squares. The error bars indicate the standard deviation from 2 to 6 independent experiments for the cells carrying the inducible Notch1 (NERT). Myf5 (G) is expressed at a very low level in undifferentiated cells (ESCs, ESCe, ESCm). Calculated inductions that do not reach significance above background levels (grey area) are indicated by an asterisk.