Activated Notch1 Target Genes during Embryonic Cell Differentiation Depend on the Cellular Context and Include Lineage Determinants and Inhibitors (original) (raw)
Figure 11
Expression of Notch1 target genes is altered in RBP-J deficient ES cells.
mRNA levels were measured by qPCR in ES cells cultured in self renewal medium (ESCs). The error bars indicate the standard deviation from 3 independent experiments. (A) Expression of potentially direct Notch1 target genes is increased in RBP-J deficient ES cells. Fold expression in relation to the expression of RBP-J +/+ D3 cells is shown. (B) The increased expression of Notch1 target genes in RBP-J -/- ES cells is repressed by exogenous RBP-J expression and further increased by a transcriptionally active form of RBP-J (RBP-J-VP16). Fold expression in relation to the expression of cells transfected with the control vector is shown.