Visualizing the Distribution of Synapses from Individual Neurons in the Mouse Brain (original) (raw)
Figure 2
Dox-dependent gene activation.
(A–E) Representative confocal images of labeled cerebellar GCs from P30 triple transgenic mouse (ZtTA/wt; TRE-Bi-SG-T/wt; β-actin-CreER/wt). Tamoxifen was injected at P7 and Dox was never administered (E) or it was removed for 0 (A), 3 (B), 7 (C) or 14 (D) days. Scale bar = 25 µm. (F) Diagram of the experimental procedure described above. (G) Quantification of the average fluorescence intensity in cerebellar GC bodies indicates that the expression level of the TRE-driven transgene increases the earlier Dox is removed, reaching maximal expression by 14 days after Dox removal. Error bars represent standard deviation. “n” represents the number of analyzed cell bodies for each condition indicated. Two animals per Dox condition were used for analysis. (H) Quantification of the total number of labeled cerebellar GCs per 225×225 µm2 for each Dox condition shows no statistically significant differences between different conditions. Error bars represent standard deviation.