Stat3 Activates the Receptor Tyrosine Kinase Like Orphan Receptor-1 Gene in Chronic Lymphocytic Leukemia Cells (original) (raw)
Figure 6
STAT3-shRNA downregulates ROR1 expression and Ror1 protein levels and ROR1-siRNA downregulates ROR1 and induces the apoptosis.
A. A 67% infection efficiency of retroviral STAT3-shRNA into CLL cells from a patient was obtained. B. qRT-PCR of these CLL cells demonstrates that STAT3-shRNA downregulated mRNA levels of ROR1, STAT3, and the Stat3-regulated genes Bcl2, Bcl-XL, Cyclin D1, c-Myc, p21, and Pim1. S18 mRNA (control) was not affected. C. STAT3-shRNA, but not the empty vector, downregulates Stat3 and Ror1 protein levels. D. Left upper panel: ROR1-siRNA downregulated ROR1 mRNA levels. qRT-PCR results are depicted. GAPDH-siRNA was used as control. Right upper corner: ROR1-siRNA, but not GAPDH-siRNA, downregulates Ror1 protein levels. Lower panel: Knock-down of ROR1 induces apoptosis. ROR1-siRNA increased CLL cell apoptosis rate of from 19% (GAPDH-siRNA control) to 39.9% (ROR1-siRNA).