Membrane Invaginations Reveal Cortical Sites that Pull on Mitotic Spindles in One-Cell C. elegans Embryos (original) (raw)
Figure 1
Invaginations imaged by spinning disc confocal and SPIM.
A, Embryos expressed PH::GFP as a membrane marker were subject to the indicated treatment. Regions with invaginations, enclosed by rectangles, are enlarged below. Invaginations are marked by yellow arrows. Accumulation of the PH::GFP signal on the centrosomes occurs in all RNAi conditions and is indicated by red arrows. Scale bars are 5 μm. B, Selective plane illumination microscopy (SPIM) of a PH::GFP embryo treated with nmy-2(RNAi). Left: z-projection of 10 slices of the indicated region of the 3D image. The original image was reduced to 8 bit and median filtered. Three invaginations (yellow arrows) all point toward the centrosome (red arrow). Scale bar is 2 μm. Right: Level-set segmentation and surface reconstruction of the region showing the conical basis (black hole) of the invaginations (yellow arrows) from the cytoplasmic side of the cell membrane.