Long-Term IGF-I Exposure Decreases Autophagy and Cell Viability (original) (raw)
Figure 8
Mouse embryo fibroblasts from IGF-I-depleted mice show increased mitochondrial mass and DNA content.
Mouse embryo fibroblasts (MEFs) were growth from IGF-I deficient mice or control animals as described in materials and methods. The relative mitochondria content in wild type and IGF-I-depleted mice measured by staining with the mitochondrial specific mitotracker green fluorescent dye as described in the Materials and Methods. A. Mean mitochondrial mass of the cell populations at passage 2 and 10 is presented as analyzed by flow cytometery. Differences between the mitochondrial mass in the IGF-I deficient mice and controls was significant (P<0.01 at passage 2 and P<0.05 at passage 10). B. Relative mitochondrial DNA content at passage 2 is presented. The experiment presented is representative of the results of 2 independent measurements on 4 DNA isolates using independent primer sets [45] that amplify mitochondrial and nuclear DNA. The difference in mitochondrial DNA content was significant (P<0.05).