Reduced Expression of IFIH1 Is Protective for Type 1 Diabetes (original) (raw)
Figure 2
Detection of aberrant splicing of IFIH1 mRNA for the two rare SNPs predicted to alter splicing.
Levels of IFIH1 pre mRNA and spliced RNA transcript were measured using qPCR assays designed to the corresponding exon to intron and exon to exon sequence. The delta Ct is calculated using the pre mRNA transcript Ct minus the mRNA transcript Ct. A larger delta Ct indicates more pre-mRNA and less mRNA transcript of IFIH1. Donor 1 and 2 are control individuals, donor 3 and 4 heterozygous for rs35337543 (IVS8+1) and donor 5 and 6 are heterozygous for rs35732034 (IVS14+1).