Matrix Rigidity Regulates Cancer Cell Growth and Cellular Phenotype (original) (raw)
Figure 6
The growth of cancer cell lines in mouse lung tissue.
A.) GFP-labeled MDA-MB-231, A549, PC-3, or mPanc96 cells were seeded into the lungs of nude mice. (Left) The number of GFP-positive cells in the lung was determined 4 hours and 14 days after injection, and the change in the number of GFP-positive cells in the lung over the 14 days was scored. * p<0.05 vs. MDA-MB-231 cells as measured by one-way ANOVA followed by Tukey's test. (Right) GFP-labeled A549 and mPanc96 cells were seeded into the lungs and the percentage of GFP-positive cells were scored at intervals over 24 hours. B.) Histology of the mouse lung at 14 days following injection of A549 cells (left panel) and mPanc96 cells (right panel). Arrows indicate micrometastases. C.) Comparison of the growth of cell lines on plastic (taken from Fig. 3A) and on 1200 Pa substrates (taken from Fig. 3B).