Toll-Like Receptor 2 Signaling Protects Mice from Tumor Development in a Mouse Model of Colitis-Induced Cancer (original) (raw)
Figure 4
Increased cell proliferation and reduced apoptosis in TLR2-deficient colons during early CAC development.
Assessment of proliferation by BrdU staining and apoptosis by TUNEL staining in proximal and distal colons from mice either treated for 14 days with the AOM-DSS regimen (Day 14) or untreated mice (Day 0) (n = 5), BrdU+ (A) and TUNEL+ (B) cells were counted in intact and well-oriented crypts. BrdU+ cells were quantified from at least 20 crypts per region from 4 different slides per animal. (C) Day 14 representative immunohistochemical stains for BrdU in colonic sections. Original magnification 100x. (D) Day 14 representative immunofluorescent stains for TUNEL stains in colonic sections. Original magnification 100x. All tests were performed using 95% confidence intervals. Data are expressed as means ± SEM. * = p<0.05, ** = p<0.01, *** = p<0.001.