Toll-Like Receptor 2 Signaling Protects Mice from Tumor Development in a Mouse Model of Colitis-Induced Cancer (original) (raw)
Figure 7
Colonic tissue from TLR2−/− mice recruit inflammatory cells that have reduced NO production and fail to mount an adequate defense against tumor growth in early CAC.
(A) Chemokines concentrations quantified by ELISA in colon homogenates in WT and TLR2−/− mice at day 14 of AOM-DSS treatment were measured by ELISA. KC (n = 6 WT, n = 9 TLR2−/−), MIP-2 (n = 6 WT, n = 9 TLR2−/−), MCP-1 (n = 6 WT, n = 4 TLR2−/−), RANTES (n = 4 WT, n = 10 TLR2−/−). (B) Immunofluorescent staining for nitrotyrosine in proximal (top panels) and distal (bottom panels) colon section of WT (left panels) and TLR2−/− (right panels) mice treated for 14 days with the AOM-DSS regimen. Original magnification 100x. (C) Quantification of nitrotyrosine positive cells normalized by area (mm2) from greater than five focus fields in at least four slides per animal (n = 5 WT and n = 5 TLR2−/−).