Single-Dose Mucosal Immunization with a Candidate Universal Influenza Vaccine Provides Rapid Protection from Virulent H5N1, H3N2 and H1N1 Viruses (original) (raw)
Figure 3
Lung T cell phenotyping.
BALB/cAnNCr mice were immunized with 5×109 particles each of A/NP-rAd and M2-rAd, or 1×1010 particles of B/NP-rAd i.n. or i.m., or were unimmunized (naïve). Lung T cells were isolated and analyzed by flow cytometry. (A) Total number of Kd-NP147–155-tetramer positive CD8+ T cells recovered from the lungs of mice, as determined by multicolor flow cytometry. Bars show mean ± SEM of 3 animals per group. (B) Phenotypic analysis of lung CD8+ T cells. Pie charts show relative proportions of central memory (CD62Lhi), effector memory (CD62Llo, CD127hi) and activated effector memory (CD62Llo, CD127lo) among tetramer positive (upper pies) and tetramer negative (lower pies) CD8+ T-cells. (C) Activation status of lung CD8+ T cells, as determined by staining for tetramer vs. CD69. Each plot shows one representative mouse per group of 3 mice assessed. Numbers in plots indicate % of CD8+ T cells per quadrant. * Indicates a statistically significant difference (P<0.05) compared to all other groups.