Single-Dose Mucosal Immunization with a Candidate Universal Influenza Vaccine Provides Rapid Protection from Virulent H5N1, H3N2 and H1N1 Viruses (original) (raw)
Figure 7
Kinetics of virus clearance following H1N1 challenge.
BALB/cAnNCr mice were immunized with 5×109 particles each of A/NP-rAd and M2-rAd, or 1×1010 particles of B/NP-rAd i.n. or i.m. and challenged with 104 TCID50 (∼100 LD50) of A/FM one month later. Lung virus titers were measured by TCID50 at the indicated time points, and are expressed as geometric mean titer ± SEM of 3 mice per group per time point, except for day 7 in the B/NP-rAd i.m. group and day 10 in the B/NP-rAd i.n. group where only 1 mouse remained alive (indicated by †). The dashed line shows limit of detection. * indicates a statistically significant difference (P<0.001) compared to all other groups at the same time point.