Specified Species in Gingival Crevicular Fluid Predict Bacterial Diversity (original) (raw)
Figure 6
Bacterial species/phylotypes responsible for the difference in bacterial composition between GCF samples and PP samples.
Multivariate OPLS/2OPLS-DA model was generated for 2 classes. The score plot (Panel A) demonstrates the relationship between the 2 types of samples and between the patients. Capital letters are patient identifiers, “G” (black color) for GCF samples and “P” (red color) for PP samples. The X variables included 133 species/phylotypes identified by DNA microarray. The binary Y variable defines the group membership to GCF or PP samples. Ellipse: Hotelling T2 (0.95). PLS regression analysis (Panel B) indicated the extent each variable positively or negatively contributed to GCF sample membership and vice versa to PP sample membership. The coefficients are statistically significant when the error bars do not cross the 0 line. The model explained 99% of the variation of Y and, according to cross validation, predicted 70% of the variation of Y. X variables were scaled and centered and Y variables scaled. The confidence intervals (95%) were derived from jack knifing. GCF: gingival crevicular fluid; PP: paper point.