Expression of Versican 3′-Untranslated Region Modulates Endogenous MicroRNA Functions (original) (raw)
Figure 1
Expression of versican 3′UTR reduces cell proliferation and tumor growth.
(a) Mouse breast carcinoma cells 4T1 was transfected with a fragment of versican 3′UTR (700 bp) or a control vector. Pooled cells were obtained. RNA was isolated from the pooled cells and the expression of 3′UTR was confirmed by RT-PCR. (b) Cell proliferation assays were performed in cells transfected with the control vector or the 3′UTR in low serum conditions (1.5% FBS) by cell counting after trypan blue staining. *, P<0.05. Error bars indicate SD (n = 5). (c) Cell cycle analysis by FACS confirmed that the vector-transfected cells had greater population of G2/M cells than the VerUTR-transfected cells. Representative data is shown. (n = 3). (d) The cells were injected subcutaneously into BALB/c mice. Tumor size was recorded weekly and tumor growth curve was obtained for a period of four weeks. Asterisks indicate significance. *, P<0.05. Error bars indicate SEM (n = 3).