Expression of Versican 3′-Untranslated Region Modulates Endogenous MicroRNA Functions (original) (raw)
Figure 2
Expression of versican 3′UTR reduces Ki67 but enhances PTEN expression.
(a) The tumors were sectioned and stained for Ki67 expression. The vector tumors showed higher levels of Ki67 staining (arrows). scale bars, 100 µm. (b) The tumor sections were stained for PTEN expression. Increased PTEN expression was detected in the 3′UTR tumors. scale bars, 100 µm. (c) PTEN expression was higher in the areas of live cells (LC) adjacent to the areas of dead cells (DC), which was more evident in the versican 3′UTR tumors than in the vector tumors. scale bars, 100 µm. (d) The tumor lysates were subject to western blot analysis, probed with anti-PTEN, anti-Rb1, and anti-actin antibodies. There was significant elevation of Rb1 and PTEN levels in the 3′UTR tumors compared with the vector control.